种属

人

别称

UMUC-3; UM-UC3; UMUC3; UC-3; University of Michigan-Urothelial Carcinoma-3

组织来源

膀胱

疾病

移行细胞癌

传代比例/细胞消化

1:2传代，消化2-3分钟

完全培养基配置

MEM培养基；10%胎牛血清；1%双抗

简介

Derived from a urinary bladder transitional cell carcinoma from a human male. These cells are tumourigenic in nude mice when 10E7 cells are inoculated subcutaneously. Hypertriploid. The modal chromosome number was 80, occurring in 42% of cells. Cells with 78 chromosomes also occurred at a high frequency. The rate of cells with higher ploidies was 2.5%. There were 30 or more marker chromosomes in each cell. They included der(1)t (1;?) (p32;?), ?t(1p5p), i(3q), t(7q14q), ?t(2p3p) and others. The X and N3 had single copy per cell, and others were generally two or three copies per cell.

形态

上皮细胞样

生长特征

贴壁生长

倍增时间

每周 2 至 3 次

致瘤性

Yes, Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells.

STR

Amelogenin: X CSF1PO: 10,11 D13S317: 8 D16S539: 8,9 D5S818: 12 D7S820: 8,9 THO1: 6,9 TPOX: 10

vWA: 17

培养条件

气相：空气，95%；二氧化碳，5%。 温度：37摄氏度，培养箱湿度为70%-80%。

冻存条件

冻存液：90%FBS，DMSO 10%,

或使用非程序冻存液：官网货号SH-H040

保藏机构  

ATCC; CRL-1749 

产品使用

仅限于科学研究，不可作为动物或人类疾病的治疗产品使用。